Difference between revisions of "Part:BBa C0083:Experience"

Revision as of 12:37, 17 October 2014

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Applications of BBa_C0083

User Reviews

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iGEM Dundee 2011

We found the sequence of this part to be correct as listed and modified it by the addition of a microcompartment targeting domain and an HA epitope tag (as BBa_K562017). This will allow better monitoring of production of the protein. We were able to detect production of this fusion in E. coli using an anti-HA monoclonal antibody. We did not get the chance to test fumarate and/or ammonia production in a host cell producing this part.

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Review number not understood iGEM Nagahama_2014

BBa_C0083 encodes aspartase with LVA tag, that tag renders protein susceptible to the action of tail-specific proteases. We removes LVA tag in BBa_C0083 resulting to new parts BBa_K1342002 And we detected and quantified of aspartic acid synthesized by E. coliJM109/BBa_K1342001 by TLC.

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-BBa_C0083 encodes aspartase with LVA tag, that tag renders protein susceptible to the action of tail-specific proteases. We removes LVA tag in BBa_C0083 resulting to new parts [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1342001 BBa_K1342001]
+BBa_C0083 encodes aspartase with LVA tag, that tag renders protein susceptible to the action of tail-specific proteases. We removes LVA tag in BBa_C0083 resulting to new parts [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1342002 BBa_K1342002]
 And we detected and quantified of aspartic acid synthesized by  <i>
 E. coli</i>JM109/[https://parts.igem.org/wiki/index.php?title=Part:BBa_K1342001 BBa_K1342001] by TLC.