Difference between revisions of "Part:BBa K660004:Experience"

 
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<partinfo>BBa_K660004 AddReview number</partinfo>
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<I>Exeter University 2014</I>
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As part of the Exeter 2014 project iLOV was used as a reporter in two constructs. iLOV was used to test the effectiveness of a NemR upstream intergenic region (https://parts.igem.org/Part:BBa_K1398005) in part BBa_1398004 (https://parts.igem.org/Part:BBa_K1398004). Part BBa_1398007 (https://parts.igem.org/Part:BBa_K1398007) used iLOV to test the effectiveness of a NemR recognizing promoter, part BBa_1398008 (https://parts.igem.org/Part:BBa_1398008).
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(Full article available at:http://2014.igem.org/Team:Exeter/Detection#TheNemRpromoterconstruct%28BBa_K1398007%29)
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As part of their project the fluorescence topography of iLOV was mapped. The response of iLOV to a low power light source was also explored. The fluorescence response of iLOV was greatest when excited with photons of wavelength 449±1nm whilst photon emissions where detected at a wavelength of 494±2nm.
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https://static.igem.org/mediawiki/2014/e/e7/Exeter2014_iLOV_ResizedBroad.png
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(Full article available at: http://2014.igem.org/Team:Exeter/iLOVCharacterisation)
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Revision as of 12:33, 17 October 2014

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Applications of BBa_K660004

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UNIQ71533ebbcfa2fbd0-partinfo-00000000-QINU

No review score entered. Exeter University 2014

As part of the Exeter 2014 project iLOV was used as a reporter in two constructs. iLOV was used to test the effectiveness of a NemR upstream intergenic region (https://parts.igem.org/Part:BBa_K1398005) in part BBa_1398004 (https://parts.igem.org/Part:BBa_K1398004). Part BBa_1398007 (https://parts.igem.org/Part:BBa_K1398007) used iLOV to test the effectiveness of a NemR recognizing promoter, part BBa_1398008 (https://parts.igem.org/Part:BBa_1398008).

(Full article available at:http://2014.igem.org/Team:Exeter/Detection#TheNemRpromoterconstruct%28BBa_K1398007%29)

As part of their project the fluorescence topography of iLOV was mapped. The response of iLOV to a low power light source was also explored. The fluorescence response of iLOV was greatest when excited with photons of wavelength 449±1nm whilst photon emissions where detected at a wavelength of 494±2nm.

Exeter2014_iLOV_ResizedBroad.png

(Full article available at: http://2014.igem.org/Team:Exeter/iLOVCharacterisation)

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UNIQ71533ebbcfa2fbd0-partinfo-00000002-QINU