Difference between revisions of "Part:BBa K1462150"
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<partinfo>BBa_K1462150 short</partinfo> | <partinfo>BBa_K1462150 short</partinfo> | ||
− | 1 | + | This part is constructed as a control group to identify our enzymes have been directed to the mitochondria.The GFP is fused at the C-terminus of AdhE2 to report where the enzyme located.Under the confocal microscope, we can observe the location of green fluorescence, thus to confirm the exact subcellular localization of target proteins. |
+ | [[File:GAG.jpg|200px|left|frame|Figure 1. <b>Enzymes located in cytoplasm</b> The fluorescence indicates that Erg10 is expressed in the cytoplasm]] | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
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<partinfo>BBa_K1462150 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1462150 SequenceAndFeatures</partinfo> | ||
Revision as of 12:32, 17 October 2014
Gal1+AdhE2+GFP+ADH1
This part is constructed as a control group to identify our enzymes have been directed to the mitochondria.The GFP is fused at the C-terminus of AdhE2 to report where the enzyme located.Under the confocal microscope, we can observe the location of green fluorescence, thus to confirm the exact subcellular localization of target proteins.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 150
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3782