Difference between revisions of "Part:BBa K1433002"
| Line 4: | Line 4: | ||
<p>Bxb1 gp35 is a serine integrase and Bxb1 gp47 is an excisionase in Mycobacterium phage Bxb1. This intergrase can exclusively catalyze site-specific recombination and mediate DNA inversion from BP state to LR state. Expression of Bxb1 gp35 and gp47 at certain ratio could change DNA from LR state to BP state. | <p>Bxb1 gp35 is a serine integrase and Bxb1 gp47 is an excisionase in Mycobacterium phage Bxb1. This intergrase can exclusively catalyze site-specific recombination and mediate DNA inversion from BP state to LR state. Expression of Bxb1 gp35 and gp47 at certain ratio could change DNA from LR state to BP state. | ||
</p> | </p> | ||
| − | + | https://static.igem.org/mediawiki/parts/e/ee/ZJU_int-xis-bplr.gif | |
<p><b><big>Composition:</big></b><br /> | <p><b><big>Composition:</big></b><br /> | ||
Latest revision as of 12:24, 17 October 2014
attL-J23110-attR
Bxb1 gp35 is a serine integrase and Bxb1 gp47 is an excisionase in Mycobacterium phage Bxb1. This intergrase can exclusively catalyze site-specific recombination and mediate DNA inversion from BP state to LR state. Expression of Bxb1 gp35 and gp47 at certain ratio could change DNA from LR state to BP state.
Composition:
- Promoter (reverse): BBa_J23110, a middle-ground Bacterial constitutive promoter.
- attL and attR sites: Recognition sites for Bxb1 gp35, Mycobacterium Phage Bxb1 DNA integrase.
This part promotes the transcription and translation of the upstream genes. When it is treated with both of Bxb1 gp35 and gp47 at certain ratio, downstream gene will be expressed.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 51
Illegal NheI site found at 74 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 24
Illegal BsaI.rc site found at 107