Difference between revisions of "Part:BBa K1462140:Design"
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[[File:GCG.jpg|200px|left|frame|Figure 1. <b>Enzymes located in cytoplasm</b> The fluorescence indicates that Erg10 is expressed in the cytoplasm]]
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===Source===
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Ccr is from Streptomyces collinus.,the other elements is provided by official.
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===References===
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Eric J Steen, et al. (2008). Metabolic engineering of Saccharomyces cerevisiae for theproduction of n-butanol, Microbial Cell Factories, 7:36.
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Revision as of 12:20, 17 October 2014

Gal1+Ccr+GFP+ADH1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1203
    Illegal BsaI.rc site found at 2549


Design Notes

This part is constructed as a control group to identify our enzymes have been directed to the mitochondria.The GFP is fused at the C-terminus of Ccr to report where the enzyme located.Under the confocal microscope, we can observe the location of green fluorescence, thus to confirm the exact subcellular localization of target proteins.


Figure 1. Enzymes located in cytoplasm The fluorescence indicates that Erg10 is expressed in the cytoplasm