Difference between revisions of "Part:BBa K1342002"
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+ | [https://parts.igem.org/Part:BBa_C0083 BBa_C0083] encodes aspartase with LVA tag, that tag renders protein susceptible to the action of tail-specific proteases. | ||
+ | We removes LVA tag in [https://parts.igem.org/Part:BBa_C0083 BBa_C0083]. | ||
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AspA is coding sequence of aspartase carries out reversible conversion of L-aspartate to fumarate and ammonia. | AspA is coding sequence of aspartase carries out reversible conversion of L-aspartate to fumarate and ammonia. | ||
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Revision as of 12:12, 17 October 2014
AspA LVA(-)
BBa_C0083 encodes aspartase with LVA tag, that tag renders protein susceptible to the action of tail-specific proteases.
We removes LVA tag in BBa_C0083.
AspA is coding sequence of aspartase carries out reversible conversion of L-aspartate to fumarate and ammonia.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 72
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 964
Illegal AgeI site found at 493
Illegal AgeI site found at 1345 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1004
TLC Assay
![](/wiki/images/4/4a/TLC_20140929_.jpg)
fig.1
Analysis of aspartic acid synthesized by E. coli JM109/BBa_K1342001 by thin-layer chromatography (TLC) Lane: 1, 2 no addition into culture (diluted 1:50 and 1:100 by synthesis medium, respectively) 3, additon of cadmium ion (250μM) 4, 5, 6 (diluted 1:50, 1:100 and 1:200 by synthesis medium, respectively)
Analysis of aspartic acid synthesized by E. coli JM109/BBa_K1342001 by thin-layer chromatography (TLC) Lane: 1, 2 no addition into culture (diluted 1:50 and 1:100 by synthesis medium, respectively) 3, additon of cadmium ion (250μM) 4, 5, 6 (diluted 1:50, 1:100 and 1:200 by synthesis medium, respectively)