Difference between revisions of "Part:BBa K1326008"
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Composite part comprised of genes encoding Magnesium chelatase subunits: [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1080000 ChlI1], [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1080002 ChlD], and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1080003 GUN4].
 
Composite part comprised of genes encoding Magnesium chelatase subunits: [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1080000 ChlI1], [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1080002 ChlD], and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1080003 GUN4].
 
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<br>[[File:pSB1C3_Operon 1.png]]
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<br> '''''Figure 1:''''' Operon 1 in the chlorophyll biosynthesis pathway.
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===Usage and Biology===
 
===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1326008 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1326008 SequenceAndFeatures</partinfo>
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[[File:GEL_Operon 1 Mg-chelatase.png]]
 
[[File:GEL_Operon 1 Mg-chelatase.png]]
 
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Figure 1: Single and double restriction digests of Operon 1. Single digest shows total plasmid length of 7.59 kB. Double digest shows Operon 1 fragment length of 4.39 kB, and BioBrick plasmid backbone length at 2.20 kB.
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Figure 2: Single and double restriction digests of Operon 1. Single digest shows total plasmid length of 7.59 kB. Double digest shows Operon 1 fragment length of 4.39 kB, and BioBrick plasmid backbone length at 2.20 kB.

Revision as of 12:10, 17 October 2014

Plac + ChlI1 + ChlD + GUN4

Composite part comprised of genes encoding Magnesium chelatase subunits: ChlI1, ChlD, and GUN4.



PSB1C3 Operon 1.png


Figure 1: Operon 1 in the chlorophyll biosynthesis pathway.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 2666
    Illegal NotI site found at 4213
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 439
    Illegal BglII site found at 1095
    Illegal BglII site found at 1290
    Illegal BglII site found at 3371
    Illegal BglII site found at 3898
    Illegal BamHI site found at 487
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1946
    Illegal NgoMIV site found at 2765
    Illegal NgoMIV site found at 3307
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

GEL Operon 1 Mg-chelatase.png
Figure 2: Single and double restriction digests of Operon 1. Single digest shows total plasmid length of 7.59 kB. Double digest shows Operon 1 fragment length of 4.39 kB, and BioBrick plasmid backbone length at 2.20 kB.