Difference between revisions of "Part:BBa K1507006"

 
 
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<partinfo>BBa_K1507006 short</partinfo>
 
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This part can convert beta carotene into beta ionone (generally known as rose fragrance when condensation is low)     
 
This part can convert beta carotene into beta ionone (generally known as rose fragrance when condensation is low)     
  
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=Testing Result :=
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===Usage and Biology===
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==Function of Rose Fragrance Generator==
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===Method:===
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====Submit beta-ionone standard and extract of our rose fragrance generator to Gas Chromatography (GC) respectively====
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===Result:===
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[[File:F test1.jpg|800px| thumb |center | Beta-ionone standard]]
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[[File:ccd4.jpg|800px| thumb |center | Beta-ionone standard]]
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====We injected the beta-ionone standard, extracted by ethyl ehanol, into Gas chromotagraphy which existed an explicit curve at 27.5 minute.====
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[[File:F test2.jpg|800px| thumb |center | Extract of rose fragrance generator]]
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[[File:ccd5.jpg|800px| thumb |center | Extract of rose fragrance generator]]
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====Then, we injected the sample which had been extracted by Ethyl ethanol also existed curves at around 27 minutes. However, the peak quality is not as good as standard.====
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===Conclusion:===
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====Success in generating rose fragrance beta-ionone from our genetic engineered '''E.coli'''====
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Latest revision as of 12:09, 17 October 2014

Rose fragrance generator (J23119-RBS34-CCD1-B0015)

This part can convert beta carotene into beta ionone (generally known as rose fragrance when condensation is low)

Testing Result :

Function of Rose Fragrance Generator

Method:

Submit beta-ionone standard and extract of our rose fragrance generator to Gas Chromatography (GC) respectively

Result:

Beta-ionone standard
Beta-ionone standard

We injected the beta-ionone standard, extracted by ethyl ehanol, into Gas chromotagraphy which existed an explicit curve at 27.5 minute.

Extract of rose fragrance generator
Extract of rose fragrance generator

Then, we injected the sample which had been extracted by Ethyl ethanol also existed curves at around 27 minutes. However, the peak quality is not as good as standard.

Conclusion:

Success in generating rose fragrance beta-ionone from our genetic engineered E.coli

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1132
    Illegal XhoI site found at 149
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1582
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 663