Difference between revisions of "Part:BBa K1507002"
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===Result:=== | ===Result:=== | ||
[[File:F test1.jpg|800px| thumb |center | Beta-ionone standard]] | [[File:F test1.jpg|800px| thumb |center | Beta-ionone standard]] | ||
| + | [[File:ccd4.jpg|800px| thumb |center | Beta-ionone standard]] | ||
====We injected the beta-ionone standard, extracted by ethyl ehanol, into Gas chromotagraphy which existed an explicit curve at 27.5 minute.==== | ====We injected the beta-ionone standard, extracted by ethyl ehanol, into Gas chromotagraphy which existed an explicit curve at 27.5 minute.==== | ||
[[File:F test2.jpg|800px| thumb |center | Extract of rose fragrance generator]] | [[File:F test2.jpg|800px| thumb |center | Extract of rose fragrance generator]] | ||
| + | [[File:ccd5.jpg|800px| thumb |center | Extract of rose fragrance generator]] | ||
====Then, we injected the sample which had been extracted by Ethyl ethanol also existed curves at around 27 minutes. However, the peak quality is not as good as standard.==== | ====Then, we injected the sample which had been extracted by Ethyl ethanol also existed curves at around 27 minutes. However, the peak quality is not as good as standard.==== | ||
Latest revision as of 12:07, 17 October 2014
Carotenoid Cleavage Dioxygenase 1 (CCD1)
Encodes a protein with 9-cis-epoxycarotenoid dioxygenase activity. The enzyme was shown to act on a variety of carotenoid including β-carotene, lutein, zeaxanthin, and all-trans-violaxanthin. When those compounds are used as substrates, the major reaction product detected is a C14 dialdehyde: 4,9-dimethyldodeca-2,4,6,8,10-pentaene-1,12-dial. The enzyme did not cleave as efficiently carotenoids containing 9-cis-double or allenic bonds.
Testing Result :
Function of Rose Fragrance Generator
Method:
Submit beta-ionone standard and extract of our rose fragrance generator to Gas Chromatography (GC) respectively
Result:
We injected the beta-ionone standard, extracted by ethyl ehanol, into Gas chromotagraphy which existed an explicit curve at 27.5 minute.
Then, we injected the sample which had been extracted by Ethyl ethanol also existed curves at around 27 minutes. However, the peak quality is not as good as standard.
Conclusion:
Success in generating rose fragrance beta-ionone from our genetic engineered E.coli
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1071
Illegal XhoI site found at 88 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1521
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 602