Difference between revisions of "Part:BBa K1321113"

Revision as of 11:52, 17 October 2014

Phytochelatin (PC) EC20 + linker-CBDcipA-linker with LacI promoter

A LacI-promoter expression construct of Synthetic phytochelatin EC20 (metal binding peptide) fused N-terminally to CBDcipA (a cellulose-binding domain), which contains an endogenous N and C-terminal linker sequence.

At present the site-directed mutagenesis for this construct is in progress to correct an illegal EcoRI site which was identified in the CBDcipA.

This construct is part of a library of fusions with cellulose binding domains which we designed to bind to cellulose and enable capture of heavy metals ([http://2014.igem.org/Team:Imperial/Functionalisation project page]). Other fusion parts with this metal binding protein can be seen in the table below:

Note that the stop codon plus 6 bp at the end of the sequence are included the RFC25 suffix which is not shown. The prefix to this part is RFC10 format.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 512
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 512
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 512
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 512
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 512
Illegal NgoMIV site found at 230
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1321113 short</partinfo>
-PhytoC+CBD10  with LacI
+A LacI-promoter expression construct of Synthetic phytochelatin EC20 (metal binding peptide) fused N-terminally to CBDcipA (a cellulose-binding domain), which contains an endogenous N and C-terminal linker sequence.
+At present the site-directed mutagenesis for this construct is in progress to correct an illegal EcoRI site which was identified in the CBDcipA.
+This construct is part of a library of fusions with cellulose binding domains which we designed to bind to cellulose and enable capture of heavy metals ([http://2014.igem.org/Team:Imperial/Functionalisation project page]). Other fusion parts with this metal binding protein can be seen in the table below: [[File:IC14-PC-part-table.PNG]]
+Note that the stop codon plus 6 bp at the end of the sequence are included the RFC25 suffix which is not shown. The prefix to this part is RFC10 format.
 <!-- Add more about the biology of this part here