Difference between revisions of "Part:BBa K1507002"

 
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__NOTOC__
 
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<partinfo>BBa_K1507002 short</partinfo>
 
<partinfo>BBa_K1507002 short</partinfo>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
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<html>
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<h4 style="color:#00A0E9">Testing Result :</h4></p>
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    <h4 style="color:gray">
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  Function of Rose Fragrance Generator</h4>
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    <h5 style="line-height:1.4em;font-size:110%;">
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    <strong style="font-size:110%">Method:</strong></p>
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    <p style="padding-bottom:30px">Submit beta-ionone standard and extract of our rose fragrance generator to Gas Chromatography (GC) respectively</p>
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    <strong style="font-size:110%;padding-bottom:30px">Result:</strong></p>
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    <figure style="padding-bottom:30px">
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<img src="images/F%20test1.jpg" width="800px">
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<br/>
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<figcaption>Beta-ionone standard</br>
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We injected the beta-ionone standard, extracted by ethyl ehanol, into Gas chromotagraphy which existed an explicit curve at 27.5 minute.
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</figcaption>
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</figure>
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      <figure style="padding-bottom:30px">
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<img src="images/F%20tset%202.jpg" width="800px">
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<br/>
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<figcaption>Extract of rose fragrance generator</br>
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Then, we injected the sample which had been extracted by Ethyl ethanol also existed curves at around 27 minutes. However, the peak quality is not as good as standard.
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</figcaption>
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<br/>
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<figcaption><b>Beta-ionone standard</b></figcaption>
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<br/>
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<img src="images/cc4.png" width="600px" style="padding-bottom:30px">
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<br/>
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<figcaption><b>Extract of rose fragrance generator</b></figcaption>
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<br/>
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<img src="images/ccd5.png" width="600px">
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</figure>
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  <strong style="font-size:110%" id="back" id="back">Conclusion:</strong></p>
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Success in generating rose fragrance beta-ionone from our genetic engineered <i>E.coli</i>.</p>
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</html>
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 11:24, 17 October 2014

Carotenoid Cleavage Dioxygenase 1 (CCD1)

Encodes a protein with 9-cis-epoxycarotenoid dioxygenase activity. The enzyme was shown to act on a variety of carotenoid including β-carotene, lutein, zeaxanthin, and all-trans-violaxanthin. When those compounds are used as substrates, the major reaction product detected is a C14 dialdehyde: 4,9-dimethyldodeca-2,4,6,8,10-pentaene-1,12-dial. The enzyme did not cleave as efficiently carotenoids containing 9-cis-double or allenic bonds.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1071
    Illegal XhoI site found at 88
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1521
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 602