Difference between revisions of "Part:BBa K1321106"
Line 3: Line 3:
  
 
A T7-promoter expression construct of Synthetic phytochelatin EC20 (metal binding peptide) fused C-terminally to CBDcipA (a cellulose-binding domain), which contains an endogenous N and C-terminal linker sequence.
 
A T7-promoter expression construct of Synthetic phytochelatin EC20 (metal binding peptide) fused C-terminally to CBDcipA (a cellulose-binding domain), which contains an endogenous N and C-terminal linker sequence.
 +
 +
At present the site-directed mutagenesis for this construct is in progress to correct an illegal EcoRI site which was identified in the CBDcipA.
  
 
This construct is part of a library of fusions with cellulose binding domains which we designed to bind to cellulose and enable capture of heavy metals ([http://2014.igem.org/Team:Imperial/Functionalisation project page]). Other fusion parts with this metal binding protein can be seen in the table below: [[File:IC14-PC-part-table.PNG]]
 
This construct is part of a library of fusions with cellulose binding domains which we designed to bind to cellulose and enable capture of heavy metals ([http://2014.igem.org/Team:Imperial/Functionalisation project page]). Other fusion parts with this metal binding protein can be seen in the table below: [[File:IC14-PC-part-table.PNG]]

Revision as of 11:16, 17 October 2014

Linker-CBDcipA-linker + Phytochelatin (PC) EC20 with T7 promoter

A T7-promoter expression construct of Synthetic phytochelatin EC20 (metal binding peptide) fused C-terminally to CBDcipA (a cellulose-binding domain), which contains an endogenous N and C-terminal linker sequence.

At present the site-directed mutagenesis for this construct is in progress to correct an illegal EcoRI site which was identified in the CBDcipA.

This construct is part of a library of fusions with cellulose binding domains which we designed to bind to cellulose and enable capture of heavy metals ([http://2014.igem.org/Team:Imperial/Functionalisation project page]). Other fusion parts with this metal binding protein can be seen in the table below: IC14-PC-part-table.PNG

Note that the stop codon plus 6 bp at the end of the sequence are included the RFC25 suffix which is not shown. The prefix to this part is RFC10 format.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 206
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 206
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 206
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 206
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 206
    Illegal NgoMIV site found at 53
  • 1000
    COMPATIBLE WITH RFC[1000]