Difference between revisions of "Part:BBa K1321100"

Revision as of 10:44, 17 October 2014

Phytochelatin (PC) EC20 fused to linker-dCBD with T7 promoter

A T7 promoter expression construct of synthetic phytochelatin EC20 (metal binding peptide) fused N-terminally to dCBD (a cellulose-binding domain), which contains an endogenous N-terminal linker sequence.

This construct is part of a library of fusions with cellulose binding domains which we designed to bind to cellulose and enable capture of heavy metals ([http://2014.igem.org/Team:Imperial/Functionalisation project page]). Other fusion parts with this metal binding protein can be seen in the table below:

Note that the start and stop codon, plus 6 bp either side of the sequence, are included the RFC25 prefix and suffix which is not shown.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 53
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1321100 short</partinfo>
-Phytochelatin EC(20) +CBD2 with T7. TBC
+A T7 promoter expression construct of synthetic phytochelatin EC20 (metal binding peptide) fused N-terminally to dCBD (a cellulose-binding domain), which contains an endogenous N-terminal linker sequence.
+This construct is part of a library of fusions with cellulose binding domains which we designed to bind to cellulose and enable capture of heavy metals ([http://2014.igem.org/Team:Imperial/Functionalisation project page]). Other fusion parts with this metal binding protein can be seen in the table below: [[File:IC14-PC-part-table.PNG]]
+Note that the start and stop codon, plus 6 bp either side of the sequence, are included the RFC25 prefix and suffix which is not shown.
 <!-- Add more about the biology of this part here