Difference between revisions of "Part:BBa K1462250:Design"
 
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===Design Notes===
 
===Design Notes===
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The n-butanol pathway in the cytoplasm is constructed as a control group to identify the effectiveness of the pathway directed to the mitochondria.AdhE2,originated from Clostridium beijerinckii, expresses butyraldehyde dehydrogenase and butanol dehydrogenase, which converts BtCoA to butyraldehyde, butyraldehyde to butanol, the fifth step and the last step of the n-butanol pathway.This part is constructed in pUC57 individually at the beginning and it is assembled with Ccr(BBa_K1462240) in plasmid 352 ,which is uracil auxotroph.
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The n-butanol pathway in the cytoplasm is constructed as a control group to identify the effectiveness of the pathway directed to the mitochondria.AdhE2,originated from Clostridium beijerinckii, expresses butyraldehyde dehydrogenase and butanol dehydrogenase, which converts BtCoA to butyraldehyde, butyraldehyde to butanol, the fifth step and the last step of the n-butanol pathway.This part is constructed in pUC57 individually at the beginning and it is assembled with Ccr(BBa_K1462240) in plasmid 352 ,which is uracil auxotroph. [[File:pathway.png|100px|left|frame|Figure 1. The n-butanol biosynthetic pathway. Enzymes are from these organisms: Erg10, S. cerevisiae; Hbd, Crt, AdhE2, Clostridium beijerinckii; Ccr, Streptomyces collinus.]]
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===Source===
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Clostridium beijerinckii
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===References===
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Eric J Steen, et al. (2008). Metabolic engineering of Saccharomyces cerevisiae for theproduction of n-butanol, Microbial Cell Factories, 7:36.
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Latest revision as of 09:33, 17 October 2014

Gal1+AdhE2+cyc1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The n-butanol pathway in the cytoplasm is constructed as a control group to identify the effectiveness of the pathway directed to the mitochondria.AdhE2,originated from Clostridium beijerinckii, expresses butyraldehyde dehydrogenase and butanol dehydrogenase, which converts BtCoA to butyraldehyde, butyraldehyde to butanol, the fifth step and the last step of the n-butanol pathway.This part is constructed in pUC57 individually at the beginning and it is assembled with Ccr(BBa_K1462240) in plasmid 352 ,which is uracil auxotroph.
Figure 1. The n-butanol biosynthetic pathway. Enzymes are from these organisms: Erg10, S. cerevisiae; Hbd, Crt, AdhE2, Clostridium beijerinckii; Ccr, Streptomyces collinus.