Difference between revisions of "Part:BBa K1317004"

Latest revision as of 09:27, 17 October 2014

Fusion protein RFP + Elastin like polypeptide (ELP)

The ELP presents specific properties: it precipitates at high temperature. This property can be used for thermal cycling purification for instance. By successive cycles of temperature, ELP can be purified from a mix of proteins. This special property has been used to create a composite part for the iGEM registry. It is a proof of concept of the use of this part as a tool for protein purification by fusing ELP to another protein. The protein used as an example is RFP, to ease vizualisation of the process.

Link on our wiki: [http://2014.igem.org/Team:Bordeaux/Parts/BBa_K1317004 http://2014.igem.org/Team:Bordeaux/Parts/BBa_K1317004]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 312
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 894
Illegal AgeI site found at 1006
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K1317004 short</partinfo>
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 This special property has been used to create a composite part for the iGEM registry. It is a proof of concept of the use of this part as a tool for protein purification by fusing ELP to another protein.
 The protein used as an example is RFP, to ease vizualisation of the process.
+Link on our wiki: [http://2014.igem.org/Team:Bordeaux/Parts/BBa_K1317004 http://2014.igem.org/Team:Bordeaux/Parts/BBa_K1317004]
 <!-- Add more about the biology of this part here