Difference between revisions of "Part:BBa K1431401"
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Here, we engineer the type II CRISPR system to come true double strands break with gRNA in hela cells.We show that this process relies on CRISPR two specificity components:gRNA and its binding target-sequence.
 
Here, we engineer the type II CRISPR system to come true double strands break with gRNA in hela cells.We show that this process relies on CRISPR two specificity components:gRNA and its binding target-sequence.
  
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In this part, we put on a high-specificity gRNA of HIV,and it have more detail in design.
+
In this part, we put on a high-specificity gRNA of HIV with more detail in design.
  
−
In addition ,this part use efficient BbsI restriction site.
+
In addition ,this part use efficient BbsI restriction site for easily used.
  
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 09:06, 17 October 2014

One gRNA Sequence for HIV-1

This is a gRNA for the HIV-1 Virus, designed by the 2014 iGEM Team SUSTC-Shenzhen.

Here, we engineer the type II CRISPR system to come true double strands break with gRNA in hela cells.We show that this process relies on CRISPR two specificity components:gRNA and its binding target-sequence.

In this part, we put on a high-specificity gRNA of HIV with more detail in design.

In addition ,this part use efficient BbsI restriction site for easily used.

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]