Difference between revisions of "Part:BBa K1431201"

Revision as of 08:59, 17 October 2014

NLS, lead protein into the nucleus

A nuclear localization signal or sequence (NLS) is an amino acid sequence that "tags" a protein for import into the cell nucleus by nuclear transport. A protein translated with a NLS will bind strongly to importin (aka karyopherin), and, together, the complex will move through the nuclear pore.

We design two NLS which connect by dozens of nucleotides and can be inserted a coding sequence by BbsI site. The two NLS in each side of a protein may increase the probability of protein import into the cell nucleus.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 66
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 88
Illegal NgoMIV site found at 107
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K1431201 short</partinfo>
-A nuclear localization signal or sequence (NLS) is an amino acid sequence that "tags" a protein for import into the cell nucleus by nuclear transport. We design two NLS which connect by dozens of nucleotides and can be inserted a coding sequence by BbsI site. The two NLS in each side of a protein may increase the probability of protein import into the cell nucleus.
+A nuclear localization signal or sequence (NLS) is an amino acid sequence that "tags" a protein for import into the cell nucleus by nuclear transport. A protein translated with a NLS will bind strongly to importin (aka karyopherin), and, together, the complex will move through the nuclear pore.
+We design two NLS which connect by dozens of nucleotides and can be inserted a coding sequence by BbsI site. The two NLS in each side of a protein may increase the probability of protein import into the cell nucleus.
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