Difference between revisions of "Part:BBa K1431201"

 
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A nuclear localization signal or sequence (NLS) is an amino acid sequence that "tags" a protein for import into the cell nucleus by nuclear transport[wikipedia]. We design two NLS which connect by dozens of nucleotides and can be inserted a coding sequence by BbsI site. The two NLS in each side of a protein may increase the probability of protein import into the cell nucleus.
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A nuclear localization signal or sequence (NLS) is an amino acid sequence that "tags" a protein for import into the cell nucleus by nuclear transport. We design two NLS which connect by dozens of nucleotides and can be inserted a coding sequence by BbsI site. The two NLS in each side of a protein may increase the probability of protein import into the cell nucleus.
  
 
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Revision as of 08:53, 17 October 2014

NLS, lead protein into the nucleus

A nuclear localization signal or sequence (NLS) is an amino acid sequence that "tags" a protein for import into the cell nucleus by nuclear transport. We design two NLS which connect by dozens of nucleotides and can be inserted a coding sequence by BbsI site. The two NLS in each side of a protein may increase the probability of protein import into the cell nucleus.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 66
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 88
    Illegal NgoMIV site found at 107
  • 1000
    COMPATIBLE WITH RFC[1000]