Difference between revisions of "Part:BBa J31001:Design"
| Line 12: | Line 12: | ||
The Biobricks on this part are not wildtype but the cut sites are still viable. | The Biobricks on this part are not wildtype but the cut sites are still viable. | ||
| − | {| | + | {| cellspacing="2" |
| − | |- | + | |- valign="top" |
| − | | style="width: | + | | style="width:300px" | '''BioBrick Prefix'''<br>There is no <font color='red'>T spacer</font color> between the<font color='brown'> NotI site</font color> and the <font color='purple'>XbaI site</font color>. There is no <font color='lime'>G spacer</font color> between the <font color='purple'>XbaI</font> and the <u>coding region</u>. |
| − | | <font face="courier"><font color='blue'>GAATTC</font><font color='brown'>GCGGCCGC</font><font color='red'>-</font><font color='purple'>TCTAGA</font><font color='lime'>-</font></font> | + | | style="background:lightgrey" | <font face="courier"><font color='blue'>GAATTC</font><font color='brown'>GCGGCCGC</font><font color='red'>-</font><font color='purple'>TCTAGA</font><font color='lime'>-</font></font> |
| − | |- | + | |- valign="top" |
| − | | Hin coding | + | | style="width:300px" | '''Hin coding''' |
| − | | <font face="courier"><u>TGGCTACTATTGGGTATATTCGGGTGTCAACAATTGACCAAAATATCGAT<br> | + | | style="background:lightgrey" | <font face="courier"><u>TGGCTACTATTGGGTATATTCGGGTGTCAACAATTGACCAAAATATCGAT<br> |
TTACAGCGTAATGCGCTTACCAGTGCAAATTGTGACCGCATTTTTGAGGA<br> | TTACAGCGTAATGCGCTTACCAGTGCAAATTGTGACCGCATTTTTGAGGA<br> | ||
CCGTATCAGTGGCAAGATTGCAAACCGCCCCGGCCTGAAACGAGCGTTAA<br> | CCGTATCAGTGGCAAGATTGCAAACCGCCCCGGCCTGAAACGAGCGTTAA<br> | ||
| Line 24: | Line 24: | ||
GGCCGCAGCGTGAAAAACCTGGTGGCGTTAATATCAGAATTACATGAACG<br> | GGCCGCAGCGTGAAAAACCTGGTGGCGTTAATATCAGAATTACATGAACG<br> | ||
TGGAGCTCACTTCCATTCTTTAACCGATAGTATTGATACCAGTAGCGCGA<br> | TGGAGCTCACTTCCATTCTTTAACCGATAGTATTGATACCAGTAGCGCGA<br> | ||
| − | + | TGGGGCGATTCTTTTTTCATGTAATGTCAGCACTGGCCGAGATGGAGCGA<br> | |
| − | + | GAATTAATTGTCGAGCGAACCCTTGCCGGACTGGCTGCCGCCAGAGCGCAAGGACGACTGGGAGGGCG<br> | |
CCCTCGGGCGATCAACAGACATGAACAGGAACAGATTAGTCGGCTATTAGAGA<br> | CCCTCGGGCGATCAACAGACATGAACAGGAACAGATTAGTCGGCTATTAGAGA<br> | ||
AAGGCCATCCTCGGCAGCAACTAGCTATTATTTTTGGTATTGGCGTATCTACCTTA<br> | AAGGCCATCCTCGGCAGCAACTAGCTATTATTTTTGGTATTGGCGTATCTACCTTA<br> | ||
TACAGATATTTTCCGGCAAGCCGTATAAAAAAACGAATGAATAGGCCTGCTGCAAA<br> | TACAGATATTTTCCGGCAAGCCGTATAAAAAAACGAATGAATAGGCCTGCTGCAAA<br> | ||
CGACGAAAACTACGCTTTAGTAGCTTA</u></font> | CGACGAAAACTACGCTTTAGTAGCTTA</u></font> | ||
| − | |- | + | |- valign="top" |
| − | | BioBrick Suffix: | + | | style="width:300px" | '''BioBrick Suffix''': There is no <font color='tan'>T spacer</font color> between the <u>insert</u> and the <font color='green'>SpeI site</font color>. The <font color='gold'>T spacer</font color> between the <font color='green'>SpeI</font color> and the <font color='magenta'>NotI</font color> sites should be an A. The last ''C'' of the <font color='magenta'>NotI</font color> site is not conserved with the initial C from the <font color='cyan'>PstI site</font color>. The BB suffix currently has this sequence for the <font color='magenta'>NotI</font color> and <font color='cyan'>PstI</font color> sites <font color='magenta'>GCGGCCGc</font color><font color='cyan'>CTGCAG</font color> But it should have been: <font color='magenta'>GCGGCCG</font color><font color='purple'>''C''</font color><font color='cyan'>TGCAG</font color> |
| − | | <font face="courier"><font color='tan'>-</font><font color='green'>ACTAGT</font><font color='gold'>T</font><font color='magenta'>GCGGCCGC</font>''C''<font color='cyan'>TGCAG</font></font> | + | | style="background:lightgrey" | <font face="courier"><font color='tan'>-</font><font color='green'>ACTAGT</font><font color='gold'>T</font><font color='magenta'>GCGGCCGC</font>''C''<font color='cyan'>TGCAG</font></font> |
|} | |} | ||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
We compared our BioBricks with those from Tom Knight's paper,<u> Idempotent Vector Design for Standard Assembly of Biobricks</u>. As seen below | We compared our BioBricks with those from Tom Knight's paper,<u> Idempotent Vector Design for Standard Assembly of Biobricks</u>. As seen below | ||
Revision as of 19:35, 26 October 2006
DNA invertase Hin tagged with LVA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is cloned in plasmid pSB1A2.
Biobricks
The Biobricks on this part are not wildtype but the cut sites are still viable.
| BioBrick Prefix There is no T spacer between the NotI site and the XbaI site. There is no G spacer between the XbaI and the coding region. |
GAATTCGCGGCCGC-TCTAGA- |
| Hin coding | TGGCTACTATTGGGTATATTCGGGTGTCAACAATTGACCAAAATATCGAT TTACAGCGTAATGCGCTTACCAGTGCAAATTGTGACCGCATTTTTGAGGA |
| BioBrick Suffix: There is no T spacer between the insert and the SpeI site. The T spacer between the SpeI and the NotI sites should be an A. The last C of the NotI site is not conserved with the initial C from the PstI site. The BB suffix currently has this sequence for the NotI and PstI sites GCGGCCGcCTGCAG But it should have been: GCGGCCGCTGCAG | -ACTAGTTGCGGCCGCCTGCAG |
We compared our BioBricks with those from Tom Knight's paper, Idempotent Vector Design for Standard Assembly of Biobricks. As seen below
Source
Salmonella typhimurium and the the Hin part without LVA.
References
Knight, Tom. Idempotent Vector Design for Standard Assembly of Biobricks