Difference between revisions of "Part:BBa K1415003"
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<partinfo>BBa_K1415003 short</partinfo> | <partinfo>BBa_K1415003 short</partinfo> | ||
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PBAN (Pheromone Biosynthesis Activating Neuropeptide) is one kind of peptides that can activate biosynthesis of pheromones of insects we target. Once a PBAN binds with the G-protein coupled receptor on an insect’s pheromone gland, the signal send by the G-protein coupled receptor activates the kinase and phosphatase, and then kinase and phosphatase can activate enzymes that participate in the biosynthesis of insect pheromone, which will be emitted. | PBAN (Pheromone Biosynthesis Activating Neuropeptide) is one kind of peptides that can activate biosynthesis of pheromones of insects we target. Once a PBAN binds with the G-protein coupled receptor on an insect’s pheromone gland, the signal send by the G-protein coupled receptor activates the kinase and phosphatase, and then kinase and phosphatase can activate enzymes that participate in the biosynthesis of insect pheromone, which will be emitted. | ||
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Control: Bacillus thuringiensis, a widely available caterpillar-killing bacterium,is a very effective control for climbing cutworms as well as for the surface feeders. | Control: Bacillus thuringiensis, a widely available caterpillar-killing bacterium,is a very effective control for climbing cutworms as well as for the surface feeders. | ||
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| + | Peptide Sequence： LADDTPATPADQEMYRPDPEQIDSRTKYFSPRL | ||
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[[File:Agrotis ipsilon.png|300px|link=|frameless|left]] | [[File:Agrotis ipsilon.png|300px|link=|frameless|left]] | ||
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Revision as of 06:32, 17 October 2014
PBAN (Agrotis ipsilon)
PBAN (Pheromone Biosynthesis Activating Neuropeptide) is one kind of peptides that can activate biosynthesis of pheromones of insects we target. Once a PBAN binds with the G-protein coupled receptor on an insect’s pheromone gland, the signal send by the G-protein coupled receptor activates the kinase and phosphatase, and then kinase and phosphatase can activate enzymes that participate in the biosynthesis of insect pheromone, which will be emitted.
So,if we ligate the constitutive promoter and ribosome binding site,we can make our E.coli produce our special peptides constantly.
Target insect:Black cutworm (Agrotis ipsilon)
Spread: This Caterpillar can be found, as various species, throughout North America.
Characteristics: Cutworms common in Georgia fields are black (Agrotis ipsilon (Ashmed)), granulate (Agrotis subterranea (Fabricius)) and variegated cutworms (Peridroma saucia(Hubner)). These are moths in the family Noctuidae. Full-grown cutworm larvae are 1.5 to 2 inches long. Coloration will vary among species, but all tend to be stout-bodied caterpillars with four sets of prolegs. They have the tendency to curl into a ball when disturbed.
Damage: Almost any plant can be attacked in the seedling stage. Cotton and certain vegetables sometimes have stand reductions. Climbing cutworms, such as the granulate cutworm, can be serious foliage feeders on some crops such as peanuts.
Control: Bacillus thuringiensis, a widely available caterpillar-killing bacterium,is a very effective control for climbing cutworms as well as for the surface feeders.
Peptide Sequence： LADDTPATPADQEMYRPDPEQIDSRTKYFSPRL
Peptide Sequence： LADDTPATPADQEMYRPDPEQIDSRTKYFSPRL
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]