Difference between revisions of "Part:BBa K1391014"

Revision as of 06:19, 17 October 2014

pENTR_LilrB2_TCS_Gal4VP16

LilrB2 fused to the recognition sequence (cleavage site) for the tobacco etch virus (TEV) protease, and the Gal4-VP16 transactivator.

When the TEV cleavage site is cleaved, it releases Gal4-VP16, which can activate the UAS-GAL4 promoter.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 603
Illegal XbaI site found at 529
Illegal PstI site found at 1549
Illegal PstI site found at 1571
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 603
Illegal PstI site found at 1549
Illegal PstI site found at 1571
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 603
Illegal BamHI site found at 215
Illegal XhoI site found at 2276
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 603
Illegal XbaI site found at 529
Illegal PstI site found at 1549
Illegal PstI site found at 1571
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 603
Illegal XbaI site found at 529
Illegal PstI site found at 1549
Illegal PstI site found at 1571
Illegal NgoMIV site found at 820
Illegal NgoMIV site found at 1513
Illegal AgeI site found at 352
Illegal AgeI site found at 852
Illegal AgeI site found at 1339
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 2195
Illegal SapI.rc site found at 192

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1391014 short</partinfo>
@@ Line 5: / Line 4: @@
 LilrB2 fused to the recognition sequence (cleavage site) for the tobacco etch virus (TEV) protease, and the Gal4-VP16 transactivator.
+When the TEV cleavage site is cleaved, it releases Gal4-VP16, which can activate the UAS-GAL4 promoter.
 <!-- Add more about the biology of this part here