Difference between revisions of "Part:BBa K1480002"
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This part is regulated by the promoter ptet (R0040) and codifies RFP/mCherry protein generator (K081014). It was constructed using 10 assembly.  
 
This part is regulated by the promoter ptet (R0040) and codifies RFP/mCherry protein generator (K081014). It was constructed using 10 assembly.  
  
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== Usage ==
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== Usage and Biology ==
  
 
It is a reporter protein that switch off when the repressor TetR binds to ptet.
 
It is a reporter protein that switch off when the repressor TetR binds to ptet.

Latest revision as of 06:13, 17 October 2014

This part is regulated by the promoter ptet (R0040) and codifies RFP/mCherry protein generator (K081014). It was constructed using 10 assembly.

Usage and Biology

It is a reporter protein that switch off when the repressor TetR binds to ptet.

Results

It is shown in red the relative fluorescence of clones without repressor. The blue bars are for the same clones regulated by the TetR generator K145201. The mean and standard deviation for red bars are 4045 and 226 and for the blue bars are 128 and 2500.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 638
    Illegal AgeI site found at 750
  • 1000
    COMPATIBLE WITH RFC[1000]