Difference between revisions of "Part:BBa K1480005"

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Here are the links for the other parts used.
 
Here are the links for the other parts used.
−
  [[Link:https://parts.igem.org/Part:BBa_K1140003|K1140003]]  
+
  [[Link:http://www.parts.igem.org/Part:BBa_K1140003|K1140003]]  
 
  [[Link:https://parts.igem.org/Part:BBa_K1480003|K1480003]]  
 
  [[Link:https://parts.igem.org/Part:BBa_K1480003|K1480003]]  
 
  [[Link:https://parts.igem.org/Part:BBa_K145201|k145201]]  
 
  [[Link:https://parts.igem.org/Part:BBa_K145201|k145201]]  

Revision as of 05:26, 17 October 2014

TetR generator (Promoter J23111 )

This is a TetR protein generator regulated by the constitutive promoter J23111.

The protein has a LVA tag for degradation.

Usage and Biology

It can be used as a constitutive generator of TetR to prove the function of a gene (or sequence) regulated by tet promoter.

Results

It is shown the effect of 4 different TetR generators on a mCherry gene (K1140003) that is down regulated by ptet. To the right side are listed the names of the generators.

Here are the links for the other parts used. 

K1140003 
K1480003 
k145201 
K1480004 
K1480005

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]