Difference between revisions of "Part:BBa K1431101"
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Tet-On(Tetracycline-Controlled Transcriptional Activation,also known as rtTA2<sup>S</sup>-M2) is a system of inducible gene expression systems for mammalian cells. Tet-On 3G (also known as rtTA-V16[citation needed]) is similar to Tet-On but was derived from rtTA2<sup>S</sup>-S2 rather than rtTA2<sup>S</sup>-M2. The Tet-On 3G protein has 5 amino acid differences compared to Tet-On which appear to increase its sensitivity to Dox even further. Tet-On 3G is sensitive to 100-fold less Dox and is 7-fold more active than the original Tet-On.<br> | Tet-On(Tetracycline-Controlled Transcriptional Activation,also known as rtTA2<sup>S</sup>-M2) is a system of inducible gene expression systems for mammalian cells. Tet-On 3G (also known as rtTA-V16[citation needed]) is similar to Tet-On but was derived from rtTA2<sup>S</sup>-S2 rather than rtTA2<sup>S</sup>-M2. The Tet-On 3G protein has 5 amino acid differences compared to Tet-On which appear to increase its sensitivity to Dox even further. Tet-On 3G is sensitive to 100-fold less Dox and is 7-fold more active than the original Tet-On.<br> | ||
Target cells that express the Tet-On 3G transactivator protein and contain a gene of interest (GOI) under the control of a TRE3G promoter (P<sub>TRE3G</sub>) will express high levels of your GOI, but only when cultured in the presence of doxycycline (Dox), which is a synthetic tetracycline derivative(the mechanism of Tet-ON 3G system show below). And the Dox concentrations required for induction of Tet-On Systems are far below cytotoxic levels for either cell culture or transgenic studies. | Target cells that express the Tet-On 3G transactivator protein and contain a gene of interest (GOI) under the control of a TRE3G promoter (P<sub>TRE3G</sub>) will express high levels of your GOI, but only when cultured in the presence of doxycycline (Dox), which is a synthetic tetracycline derivative(the mechanism of Tet-ON 3G system show below). And the Dox concentrations required for induction of Tet-On Systems are far below cytotoxic levels for either cell culture or transgenic studies. | ||
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| + | [https://static.igem.org/mediawiki/2014/3/37/SUSTC-Shenzhen_Tet_On_3G.png] | ||
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Revision as of 05:24, 17 October 2014
TetOn-3G, an ideal controller of mammalian gene expression with TRE-3G promoter+PolyA
Tet-On(Tetracycline-Controlled Transcriptional Activation,also known as rtTA2S-M2) is a system of inducible gene expression systems for mammalian cells. Tet-On 3G (also known as rtTA-V16[citation needed]) is similar to Tet-On but was derived from rtTA2S-S2 rather than rtTA2S-M2. The Tet-On 3G protein has 5 amino acid differences compared to Tet-On which appear to increase its sensitivity to Dox even further. Tet-On 3G is sensitive to 100-fold less Dox and is 7-fold more active than the original Tet-On.
Target cells that express the Tet-On 3G transactivator protein and contain a gene of interest (GOI) under the control of a TRE3G promoter (PTRE3G) will express high levels of your GOI, but only when cultured in the presence of doxycycline (Dox), which is a synthetic tetracycline derivative(the mechanism of Tet-ON 3G system show below). And the Dox concentrations required for induction of Tet-On Systems are far below cytotoxic levels for either cell culture or transgenic studies.
![[1]](https://static.igem.org/mediawiki/2014/3/37/SUSTC-Shenzhen_Tet_On_3G.png){kind=link}
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]