Difference between revisions of "Part:BBa K1323004"

Revision as of 05:07, 17 October 2014

Hfq: Expression cassette under a xylose inducible promoter

Hfq is a protein which enhances sRNA complementation to its mRNA target - It does so by binding AU-rich and poly-A-rich sequences; sRNAs which associate with Hfq have greater target binding rate, target mRNA degradation, and half-life (Yoo et al., 2013). Species which are closely related to (and including) E. coli contain the Hfq gene in their chromosomes - other species, including Staphylococcus sp., will require this expression cassette if heightened sRNA activity is to be achieved. Illegal sites were removed and the sequence was codon optimized for expression in S. epidermidis using JCat software.

This part was DNA-Synthesized.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1476
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

References

Yoo, S, M., Na, D., Lee, S. Y. (2013). Design and use of synthetic regulatory small RNAs to control gene expression in Escherichia coli. Nature protocol 8(9), 1694-1707.

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 <partinfo>BBa_K1323004 short</partinfo>
-Hfq is a protein which enhances sRNA complementation to its mRNA target - It does so by binding AU-rich and poly-A-rich sequences; Hfq-dependent sRNAs have greater target binding rate, target mRNA degradation, and half-life (Yoo et al., 2013). Species which are closely related to (and including) E. coli contain the Hfq gene in their chromosomes - other species, including Staphylococcus sp., will require this expression cassette if heightened sRNA activity is to be achieved. Illegal sites were removed and the sequence was codon optimized for expression in S. epidermidis using JCat software.
+Hfq is a protein which enhances sRNA complementation to its mRNA target - It does so by binding AU-rich and poly-A-rich sequences; sRNAs which associate with Hfq have greater target binding rate, target mRNA degradation, and half-life (Yoo et al., 2013). Species which are closely related to (and including) E. coli contain the Hfq gene in their chromosomes - other species, including Staphylococcus sp., will require this expression cassette if heightened sRNA activity is to be achieved. Illegal sites were removed and the sequence was codon optimized for expression in S. epidermidis using JCat software.
 This part was DNA-Synthesized.