Difference between revisions of "Part:BBa K1462090"
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| − | + | To test the localization function of CoxVI, the C-terminus of CoxVI was fused to GFP. Besides,we use MitoTracker Red®CMXRos to stain the mitochondria and do an overlap with GFP. By observing the coefficient value, we can judge that if our leading peptide works.We adopted fluorescence test to have a visible look at where the fusion protein is located. The following microscope images were obtained. It was observed that green fluorescence concentrated in mitochondria stained by a specific stain, instead of diffusing in the cytosol.The reult proves that CoxVI can locate GFP into the mitochondrial matrix. | |
| + | [[G6GT2.jpg|200px|left|frame|你的文字]] | ||
Revision as of 04:30, 17 October 2014
Gal1+cox6+GFP+ADH1
To test the localization function of CoxVI, the C-terminus of CoxVI was fused to GFP. Besides,we use MitoTracker Red®CMXRos to stain the mitochondria and do an overlap with GFP. By observing the coefficient value, we can judge that if our leading peptide works.We adopted fluorescence test to have a visible look at where the fusion protein is located. The following microscope images were obtained. It was observed that green fluorescence concentrated in mitochondria stained by a specific stain, instead of diffusing in the cytosol.The reult proves that CoxVI can locate GFP into the mitochondrial matrix. 200px|left|frame|你的文字
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 150
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1325