Difference between revisions of "Part:BBa K537020:Experience"

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We want to know whether recombination will happen without the cre recombinase. So we design the device to verify. We only transform this device[fig.1] into DH5α and DH10B.At the same time, the samples were sequenced and anything was OK. In summary, we confirmed the unexpected recombination of lox71 site even if the device is in the stable E.coli strains such as the DH5α and DH10B. But the probability of its occurrence is extremely low. And it doesn’t affect the sequencing result in a short time. But as we explained in the tetR repressor protein (BBa_C0040), we don’t recommend you used the cre/lox system to achieve the toxic protein’s deletion or rolling over.
 
We want to know whether recombination will happen without the cre recombinase. So we design the device to verify. We only transform this device[fig.1] into DH5α and DH10B.At the same time, the samples were sequenced and anything was OK. In summary, we confirmed the unexpected recombination of lox71 site even if the device is in the stable E.coli strains such as the DH5α and DH10B. But the probability of its occurrence is extremely low. And it doesn’t affect the sequencing result in a short time. But as we explained in the tetR repressor protein (BBa_C0040), we don’t recommend you used the cre/lox system to achieve the toxic protein’s deletion or rolling over.
  
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[[File:HZAU2014-lox71 p1.png|400px|thumb|centre|fig.1]]
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[[File:HZAU2014-lox71 p1.png|400px|thumb|centre|fig.1:Confirmation Device]]
  
 
[[File:HZAU2014-lox71 p3.png|600px|thumb|centre]]
 
[[File:HZAU2014-lox71 p3.png|600px|thumb|centre]]

Latest revision as of 03:24, 17 October 2014


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K537020

User Reviews

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••••

HZAU-China 2014


We want to know whether recombination will happen without the cre recombinase. So we design the device to verify. We only transform this device[fig.1] into DH5α and DH10B.At the same time, the samples were sequenced and anything was OK. In summary, we confirmed the unexpected recombination of lox71 site even if the device is in the stable E.coli strains such as the DH5α and DH10B. But the probability of its occurrence is extremely low. And it doesn’t affect the sequencing result in a short time. But as we explained in the tetR repressor protein (BBa_C0040), we don’t recommend you used the cre/lox system to achieve the toxic protein’s deletion or rolling over.

fig.1:Confirmation Device
HZAU2014-lox71 p3.png
HZAU2014-lox71 p2.png

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