Difference between revisions of "Part:BBa K1362021:Design"
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===Design Notes=== | ===Design Notes=== | ||
The His-Tag allows simple purification with His-Trap Column. Furthermore a Anti 6 His-Tag Antibody can be used on a Western Blot for detection of the enzyme. | The His-Tag allows simple purification with His-Trap Column. Furthermore a Anti 6 His-Tag Antibody can be used on a Western Blot for detection of the enzyme. | ||
| + | |||
| + | For more information on the circularization see the [[Part:BBa_K1362000|Intein Circularization Construct]] | ||
===Source=== | ===Source=== | ||
Latest revision as of 02:52, 17 October 2014
RBS + Circular Xylanase with His6 linker
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 153
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 557
Illegal SapI.rc site found at 611
Design Notes
The His-Tag allows simple purification with His-Trap Column. Furthermore a Anti 6 His-Tag Antibody can be used on a Western Blot for detection of the enzyme.
For more information on the circularization see the Intein Circularization Construct
Source
See the Basic Xylanase Part