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| + | <partinfo>BBa_P0440 AddReview 3</partinfo> | ||
| + | <I>HZAU-China 2014</I> | ||
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| + | We met some problem in our construction. It seems that the overexpression of repressor protein has cytotoxicity which made troubles in our process. When we ligated tetR protein coding sequence in our circuit as the picture followed .Then we fund that recombination happened without Cre recombinase. So the promoter had been removed. It seems that the overexpression of repressor protein has cytotoxicity which made troubles in our process. Compared with the random mutation, the lox71 site is easier to delete the promoter and the bacteria without tetR protein can get a growth advantage. So, at last, there is only the bacteria without promoter in the medium. | ||
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| + | [[File:HZAU2014-tetR.png|600px]] | ||
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Latest revision as of 02:50, 17 October 2014
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_P0440
User Reviews
UNIQaa4f7fb47706c399-partinfo-00000000-QINU
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•••
HZAU-China 2014 |
We met some problem in our construction. It seems that the overexpression of repressor protein has cytotoxicity which made troubles in our process. When we ligated tetR protein coding sequence in our circuit as the picture followed .Then we fund that recombination happened without Cre recombinase. So the promoter had been removed. It seems that the overexpression of repressor protein has cytotoxicity which made troubles in our process. Compared with the random mutation, the lox71 site is easier to delete the promoter and the bacteria without tetR protein can get a growth advantage. So, at last, there is only the bacteria without promoter in the medium.
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UNIQaa4f7fb47706c399-partinfo-00000002-QINU