Difference between revisions of "Part:BBa K1323006:Experience"
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[[File:sRNA Graph.png]] | [[File:sRNA Graph.png]] | ||
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| + | ==sRNAi Silencing assay == | ||
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| + | YFP construct (pSB3K3 backbone, sarA protomer (BBa_K1323021),TIR rbs (BBa_K1323016), YFP cds(BBa_K1323010)) and sRNA constructs were co-transformed into DH5α. Colonies were streak purified and grown in LB supplemented with ampicillin. A restriction digest was run to confirm that both plasmids were in the colonies selected. Overnight cultures were prepared in triplicate for each sample. 50 µL of overnight culture was used to inoculate fresh media, and once the OD600 reached 0.5-0.6, the YFP fluorescence was measured. | ||
===Applications of BBa_K1323006=== | ===Applications of BBa_K1323006=== | ||
Revision as of 02:43, 17 October 2014
sRNAi Silencing assay
YFP construct (pSB3K3 backbone, sarA protomer (BBa_K1323021),TIR rbs (BBa_K1323016), YFP cds(BBa_K1323010)) and sRNA constructs were co-transformed into DH5α. Colonies were streak purified and grown in LB supplemented with ampicillin. A restriction digest was run to confirm that both plasmids were in the colonies selected. Overnight cultures were prepared in triplicate for each sample. 50 µL of overnight culture was used to inoculate fresh media, and once the OD600 reached 0.5-0.6, the YFP fluorescence was measured.
Applications of BBa_K1323006
User Reviews
UNIQ3de5b63ec40f2b69-partinfo-00000000-QINU UNIQ3de5b63ec40f2b69-partinfo-00000001-QINU