Difference between revisions of "Part:BBa K1323005:Experience"

 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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how you used this part and how it worked out.
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[[File:sRNA Graph.png]]
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==sRNAi Silencing assay==
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YFP construct (pSB3K3 backbone, sarA protomer (BBa_K1323021),TIR rbs (BBa_K1323016), YFP cds(BBa_K1323010)) and sRNA constructs were co-transformed into DH5α. Colonies were streak purified and grown in LB supplemented with ampicillin. A restriction digest was run to confirm that both plasmids were in the colonies selected. Overnight cultures were prepared in triplicate for each sample. 50 µL of overnight culture was used to inoculate fresh media, and once the OD600 reached 0.5-0.6, the YFP fluorescence was measured.  
  
 
===Applications of BBa_K1323005===
 
===Applications of BBa_K1323005===

Revision as of 02:41, 17 October 2014


SRNA Graph.png


sRNAi Silencing assay

YFP construct (pSB3K3 backbone, sarA protomer (BBa_K1323021),TIR rbs (BBa_K1323016), YFP cds(BBa_K1323010)) and sRNA constructs were co-transformed into DH5α. Colonies were streak purified and grown in LB supplemented with ampicillin. A restriction digest was run to confirm that both plasmids were in the colonies selected. Overnight cultures were prepared in triplicate for each sample. 50 µL of overnight culture was used to inoculate fresh media, and once the OD600 reached 0.5-0.6, the YFP fluorescence was measured.

Applications of BBa_K1323005

User Reviews

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