Difference between revisions of "Part:BBa K1462540"
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<partinfo>BBa_K1462540 short</partinfo>
 
<partinfo>BBa_K1462540 short</partinfo>
  
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Scaffold protein, with a leading peptide Tom22, can locate on the outer mitochondria membrane with the scale of GBD: SH3: PDZ==1:1:2.We  built up the scale to affirm the best scale. In this way , we tried to fix the PRK, RuBisCO, CA  to a limited space to make full use of ATP and CO2 so that we coule improve the pyruvate offering to the butanol pathway.
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Scaffold protein, with a leading peptide Tom22, can locate on the outer mitochondria membrane with the scale of GBD: SH3: PDZ==1:1:1.We  built up the scale to affirm the best scale. In this way , we tried to fix the PRK, RuBisCO, CA  to a limited space to make full use of ATP and CO2 so that we coule improve the pyruvate offering to the butanol pathway.
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<img src="https://static.igem.org/mediawiki/parts/f/fb/Rubisco.jpg"><br/>
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Figure 1.This is the pathway and the structure of the carbon capture. As we see, with the help of ATP,the RuBiSco-5P change into RuBiSco-1,5P by PRK, and then change into 3P-glyrerate by RuBiSco, and CA can help us to adjust the concentration of carbon dioxide. The x,y,z are the number of scaffold unit.
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<br/>[[File:Rubisco.jpg|200px|left|frame|Figure 1.This is the pathway and the structure of the carbon capture. As we see, with the help of ATP,the RuBiSco-5P change into RuBiSco-1,5P by PRK, and then change into 3P-glyrerate by RuBiSco, and CA can help us to adjust the concentration of carbon dioxide. The x,y,z are the number of scaffold unit.]]
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 02:06, 17 October 2014

GAL1+GBD+SH3+PDZ+PDZ+Tom22+ADH1

Scaffold protein, with a leading peptide Tom22, can locate on the outer mitochondria membrane with the scale of GBD: SH3: PDZ==1:1:1.We built up the scale to affirm the best scale. In this way , we tried to fix the PRK, RuBisCO, CA to a limited space to make full use of ATP and CO2 so that we coule improve the pyruvate offering to the butanol pathway.


Figure 1.This is the pathway and the structure of the carbon capture. As we see, with the help of ATP,the RuBiSco-5P change into RuBiSco-1,5P by PRK, and then change into 3P-glyrerate by RuBiSco, and CA can help us to adjust the concentration of carbon dioxide. The x,y,z are the number of scaffold unit.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1873
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1873
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 615
    Illegal BamHI site found at 648
    Illegal BamHI site found at 820
    Illegal BamHI site found at 1029
    Illegal BamHI site found at 1352
    Illegal BamHI site found at 1675
    Illegal XhoI site found at 801
    Illegal XhoI site found at 1010
    Illegal XhoI site found at 1333
    Illegal XhoI site found at 1656
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1873
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1873
    Illegal AgeI site found at 150
  • 1000
    COMPATIBLE WITH RFC[1000]