Difference between revisions of "Part:BBa K1321338:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1321338 short</partinfo> | <partinfo>BBa_K1321338 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
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| + | PCR was performed using BBa_I746909 as a template with the following primers: | ||
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| + | Forward Primer: 5'-TAC TAG TAG CGG CCG CTG CAG-3' | ||
| + | Reverse Primer: 5'-TTT CTC CTC TTT CTC TAG TAT CTC CC-3' | ||
===Source=== | ===Source=== | ||
| − | + | PCR template was BBa_I746909 which was miniprepped from 2014 distribution Plate 3 Well 8M | |
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Latest revision as of 01:44, 17 October 2014
T7 Expression vector
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
PCR was performed using BBa_I746909 as a template with the following primers:
Forward Primer: 5'-TAC TAG TAG CGG CCG CTG CAG-3' Reverse Primer: 5'-TTT CTC CTC TTT CTC TAG TAT CTC CC-3'
Source
PCR template was BBa_I746909 which was miniprepped from 2014 distribution Plate 3 Well 8M