Difference between revisions of "Part:BBa K1323013"

Revision as of 01:08, 17 October 2014

aadD - Kanamycin Resistance Gene Coding Sequence

This coding sequence is for the antibiotic kanamycin. The sequence of this gene is obtained from pKAN vector developed by Dr. Jeffery Bose (KUMC) and Dr. Kenneth Bayles (NUMC) (Bose et al., 2013). The working concentration of kanamycin is 250ug/mL for aadD expressed on a plasmid and 75ug/mL for aadD on chromosome (Bose et al., 2013)

Bose, J. L., Fey, P. D., and Bayles, K. W. (2013). Genetic Tools to Enhance the Study of Gene Function and Regulation in Staphylococcus aureus. Applied and Environmental Microbiology. 79 (7): 2218-2224. Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 561
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 172
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 385

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1323013 short</partinfo>
-TBA
+This coding sequence is for the antibiotic kanamycin. The sequence of this gene is obtained from pKAN vector developed by Dr. Jeffery Bose (KUMC) and Dr. Kenneth Bayles (NUMC) (Bose et al., 2013). The working concentration of kanamycin is 250ug/mL for aadD expressed on a plasmid and 75ug/mL for aadD on chromosome (Bose et al., 2013)
+Bose, J. L., Fey, P. D., and Bayles, K. W. (2013). Genetic Tools to Enhance the Study of Gene Function and Regulation in Staphylococcus aureus. Applied and Environmental Microbiology. 79 (7): 2218-2224.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===