Difference between revisions of "Part:BBa K1323009"

Revision as of 01:03, 17 October 2014

DsRed (RFP) Coding Sequence

This is the coding sequence of DsRed_T3 (DNT), a highly fluorescent mutant version of a red fluorescent protein originally found in Discosoma (corals) (Dunn et. al., 2006). It was codon optimized for Staphylococcus aureus by Bose et. al. (2013) and we had this part synthesized by Bio Basic Canada Inc.

Bose, J. L., Fey, P. D., and Bayles, K. W. (2013). Genetic Tools to Enhance the Study of Gene Function and Regulation in Staphylococcus aureus. Applied and Environmental Microbiology. 79 (7): 2218-2224.

Dunn, A. K., Millikan, D. S., Adin, D. M., Bose, J. B., and Stabb, E. V. (2006). New rfp- and pES213-Derived Tools for Analyzing Symbiotic Vibrio fisheri Reveal Patterns of Infection and lux Expression In Situ. Applied and Environmental Microbiology. 72 (1): 802-810. Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K1323009 short</partinfo>
-TBA
+This is the coding sequence of DsRed_T3 (DNT), a highly fluorescent mutant version of a red fluorescent protein originally found in Discosoma (corals) (Dunn et. al., 2006). It was codon optimized for Staphylococcus aureus by Bose et. al. (2013) and we had this part synthesized by Bio Basic Canada Inc.
+Bose, J. L., Fey, P. D., and Bayles, K. W. (2013). Genetic Tools to Enhance the Study of Gene Function and Regulation in Staphylococcus aureus. Applied and Environmental Microbiology. 79 (7): 2218-2224.
+Dunn, A. K., Millikan, D. S., Adin, D. M., Bose, J. B., and Stabb, E. V. (2006). New rfp- and pES213-Derived Tools for Analyzing Symbiotic Vibrio fisheri Reveal Patterns of Infection and lux Expression In Situ. Applied and Environmental Microbiology. 72 (1): 802-810.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===