Difference between revisions of "Part:BBa K1493601"
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Kis (antitoxin) and Kid (toxin) are part of the parD stability system of plasmid R1 acting as a pre-segregation mechanism to ensure plasmid proliferation. Kid breaks down single stranded messenger RNAs. By degradation of something as vital as messenger RNA, this toxin does not differ in function with gram positive and gram negative bacteria. In nature, Kid causes cells to divide slower to ensure that every daughter cell receives the R1 plasmid. In our system an excess of Kid will result in a bactericidal effect. Additionally, the Kis-Kid interaction has proven to be a 1:2 ratio [5, 6]. | Kis (antitoxin) and Kid (toxin) are part of the parD stability system of plasmid R1 acting as a pre-segregation mechanism to ensure plasmid proliferation. Kid breaks down single stranded messenger RNAs. By degradation of something as vital as messenger RNA, this toxin does not differ in function with gram positive and gram negative bacteria. In nature, Kid causes cells to divide slower to ensure that every daughter cell receives the R1 plasmid. In our system an excess of Kid will result in a bactericidal effect. Additionally, the Kis-Kid interaction has proven to be a 1:2 ratio [5, 6]. | ||
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+ | References |
Revision as of 22:16, 16 October 2014
Kis Antitoxin (Kis-Kid Toxin-Antitoxin System)
Kis (antitoxin) and Kid (toxin) are part of the parD stability system of plasmid R1 acting as a pre-segregation mechanism to ensure plasmid proliferation. Kid breaks down single stranded messenger RNAs. By degradation of something as vital as messenger RNA, this toxin does not differ in function with gram positive and gram negative bacteria. In nature, Kid causes cells to divide slower to ensure that every daughter cell receives the R1 plasmid. In our system an excess of Kid will result in a bactericidal effect. Additionally, the Kis-Kid interaction has proven to be a 1:2 ratio [5, 6].
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 131
- 1000COMPATIBLE WITH RFC[1000]
Functional Parameters
References