Difference between revisions of "Part:BBa K1316018:Design"
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===Source=== | ===Source=== | ||
| − | + | The CDS of csgB, as well as the RBS, contained in this part were PCRed from the iGEM BioBrick BBa_K540000 Lyon team 2011. | |
===References=== | ===References=== | ||
Latest revision as of 22:03, 16 October 2014
RBS + csgB curli-forming gene
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 516
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The part contains an RBS upstream the gene in order to ensure proper protein translation. The part was PCRed in a way that it contains the beginning of a constitutive promoter after the gene. This aims at constructing via Golden Gate Assembly the final BioBricks BBa_K1316013 and BBa_K1316014 where csgB and csgA genes are present and a constitutive promoter was placed between them.
Source
The CDS of csgB, as well as the RBS, contained in this part were PCRed from the iGEM BioBrick BBa_K540000 Lyon team 2011.