Difference between revisions of "Part:BBa K1463520"
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See [https://parts.igem.org/Part:BBa_K1463560 BBa_K1463560] and [https://parts.igem.org/Part:BBa_K1463000 BBa_K1463000] for more details. | See [https://parts.igem.org/Part:BBa_K1463560 BBa_K1463560] and [https://parts.igem.org/Part:BBa_K1463000 BBa_K1463000] for more details. | ||
| − | [[Image:reverse-rbs-rfp.jpg|thumb|500px|center|'''Fig. 1:''' Red fluorescence of colonies containing [https://parts.igem.org/Part:BBa_K1463560 BBa_K1463560] or [ | + | [[Image:reverse-rbs-rfp.jpg|thumb|500px|center|'''Fig. 1:''' Red fluorescence of colonies containing [https://parts.igem.org/Part:BBa_K1463560 BBa_K1463560] or [https://parts.igem.org/Part:BBa_I742130 BBa_I742130] reversed RBSs "downstream" of the reversed rfp gene [https://parts.igem.org/Part:BBa_K1463520 BBa_K1463520] transcribed from the PhiC31 integrase switch [https://parts.igem.org/Part:BBa_K1463000 K1463000]]] |
Revision as of 21:55, 16 October 2014
E1010 RFP in reverse
Part E1010, RFP, in reverse.
Usage and Biology
This part gave red fluorescence when assembled adjacent to the reversed Ribosome Binding Site BBa_K1463560 with transcription driven by the reverse promoter BBa_K1463501 in BBa_K1463000.
See BBa_K1463560 and BBa_K1463000 for more details.
Fig. 1: Red fluorescence of colonies containing BBa_K1463560 or BBa_I742130 reversed RBSs "downstream" of the reversed rfp gene BBa_K1463520 transcribed from the PhiC31 integrase switch K1463000
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 34
Illegal AgeI site found at 146 - 1000COMPATIBLE WITH RFC[1000]