Difference between revisions of "Part:BBa K1463520"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1463520 short</partinfo>
 
<partinfo>BBa_K1463520 short</partinfo>
Line 5: Line 4:
 
Part E1010, RFP, in reverse.
 
Part E1010, RFP, in reverse.
  
−
<!-- Add more about the biology of this part here
+
<!-- Add more about the biology of this part here -->
 
===Usage and Biology===
 
===Usage and Biology===
 +
 +
This part gave red fluorescence when assembled adjacent to the reversed Ribosome Binding Site [https://parts.igem.org/Part:BBa_K1463560 BBa_K1463560] with transcription driven by the reverse promoter [https://parts.igem.org/Part:BBa_K1463501 BBa_K1463501] in [https://parts.igem.org/Part:BBa_K1463000 BBa_K1463000].
 +
 +
See [https://parts.igem.org/Part:BBa_K1463560 BBa_K1463560] and [https://parts.igem.org/Part:BBa_K1463000 BBa_K1463000] for more details.
 +
 +
[[Image:reverse-rbs-rfp.jpg|thumb|500px|center|'''Fig. 1:''' Red fluorescence of colonies containing BBa_I742130 or K1463560 reversed RBSs downstream of rfp gene transcribed from the PhiC31 integrase switch [https://parts.igem.org/Part:BBa_K1463000 K1463000]]]
 +
  
 
<!-- -->
 
<!-- -->

Revision as of 21:48, 16 October 2014

E1010 RFP in reverse

Part E1010, RFP, in reverse.

Usage and Biology

This part gave red fluorescence when assembled adjacent to the reversed Ribosome Binding Site BBa_K1463560 with transcription driven by the reverse promoter BBa_K1463501 in BBa_K1463000.

See BBa_K1463560 and BBa_K1463000 for more details.

Fig. 1: Red fluorescence of colonies containing BBa_I742130 or K1463560 reversed RBSs downstream of rfp gene transcribed from the PhiC31 integrase switch K1463000


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 34
    Illegal AgeI site found at 146
  • 1000
    COMPATIBLE WITH RFC[1000]