Difference between revisions of "Part:BBa K1541017"

Revision as of 21:20, 16 October 2014

rhlI with optimized RBS

N-butyryl-L-Homoserine lactone or short C4-HSL

This part contains the part C0170 (RhlI), which is an autoinducer synthesis protein that produces N-butyryl-L-HSL (C4-HSL) which binds to RhlR (C0171), obtained from Pseudomonas aeruginosa. Since this part was used in a low-copy vector, RBS engineering was performed to increase expression levels of this enzyme and thus also improve the production of C4-HSL.

Usage and Biology

Quorum Sensing

RhlI is the autoinducer synthetase from Pseudomonas aeruginosa, which can synthesize the molecule N-butyryl-L-HSL (C4-HSL). C4-HSL can bind to the regulatory protein RhlR (C0171). Once C4-HSL is bound to RhlR it can activate the promoters R0071 or I14017.

RBS Engineering

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 6: / Line 6: @@
 ===Usage and Biology===
 ====Quorum Sensing====
-RhlI is the autoinducer synthetase from ''Pseudomonas aeruginosa'', which can synthesize the molecule [[AHL|''N''-butyryl-L-HSL]] (C4-HSL). C4-HSL can bind to the regulatory protein RhlR ([[Part:BBa_C0171|C0171]]).
+RhlI is the autoinducer synthetase from ''Pseudomonas aeruginosa'', which can synthesize the molecule [[AHL|''N''-butyryl-L-HSL]] (C4-HSL). C4-HSL can bind to the regulatory protein RhlR ([[Part:BBa_C0171|C0171]]). Once C4-HSL is bound to [[Part:BBa_C0171|RhlR]] it can activate the promoters [[Part:BBa_R0071|R0071]] or [[Part:BBa_I14017|I14017]].
 ====RBS Engineering====