Difference between revisions of "Part:BBa K1321368"

Revision as of 21:11, 16 October 2014

CBDclos + NiBP in RFC25 fusion format

**Important note**: Please be aware we discovered that this part is out of frame, leading to an incorrect translation and read-through of the stop-codon. This also means any subsequent fusion proteins to the C-terminal are out of frame. This is because there is a deletion of a base compared to the equivalent coding sequence from Heliobacter pylori (GenBank CP000012.1 bp1391851-1392033, reverse complement strand). This existed already in BBa_K1151001 from which we cloned our part.

This construct is part of a library of fusions with cellulose binding domains which we designed to bind to cellulose and enable capture of heavy metals. Other fusion parts with this metal binding protein can be seen in the table below:

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K1321368 short</partinfo>
-GATC
+'''**Important note**''': Please be aware we discovered that this part is out of frame, leading to an incorrect translation and read-through of the stop-codon. This also means any subsequent fusion proteins to the C-terminal are out of frame. This is because there is a deletion of a base compared to the equivalent coding sequence from ''Heliobacter pylori'' (GenBank CP000012.1 bp1391851-1392033, reverse complement strand). This existed already in BBa_K1151001 from which we cloned our part.
+This construct is part of a library of fusions with cellulose binding domains which we designed to bind to cellulose and enable capture of heavy metals. Other fusion parts with this metal binding protein can be seen in the table below: [[File:IC14-NiBP-part-table.PNG]]
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
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