Difference between revisions of "Part:BBa K1319001"

Revision as of 20:57, 16 October 2014

RFC[25]-compatible dark quencher based on K1319000 (E0030)

This part is a RFC[25] dark quencher that is based upon K1319000 (the RFC[25] version of E0030/EYFP)..

Two mutations were introduced that eliminated fluorescence:

Ganesan S, Ameer-Beg SM, Ng TT, Vojnovic B, Wouters FS. A dark yellow fluorescent protein (YFP)-based Resonance Energy-Accepting Chromoprotein (REACh) for Förster resonance energy transfer with GFP. Proc Natl Acad Sci U S A. 2006 Mar 14;103(11):4089-94. Epub 2006 Mar 6. PubMed PMID: 16537489; PubMed Central PMCID: PMC1449651. [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1449651/?report=classic PubMed Central]

Sequence and Features

Assembly Compatibility:

Revision as of 22:17, 3 September 2014 (view source) Mosthege (Talk \| contribs) m (→‎References) ← Older edit		Revision as of 20:57, 16 October 2014 (view source) AZimmermann (Talk \| contribs) Newer edit →
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−	This part is a RFC[25] dark quencher that is based upon K1319000 (the RFC[25] version of E0030/EYFP).	+	This part is a RFC[25] dark quencher that is based upon K1319000 (the RFC[25] version of E0030/EYFP)..

	Two mutations were introduced that eliminated fluorescence:		Two mutations were introduced that eliminated fluorescence: