Difference between revisions of "Part:BBa K1541017"

Revision as of 20:56, 16 October 2014

rhlI with optimized RBS

N-butyryl-L-Homoserine lactone or short C4-HSL

This part contains the part Part:BBa_C0170 C0170 (RhlI), which is an autoinducer synthesis protein that produces N-butyryl-L-HSL (C4-HSL) which binds to RhlR, (Part:BBa_C0171 C0171), obtained from Pseudomonas aeruginosa. Since this part was used in a low-copy vector, RBS engineering was performed to increase expression levels of this enzyme and thus also improve the production of C4-HSL.

Usage and Biology

Quorum Sensing

RhlI is the autoinducer synthetase from Pseudomonas aeruginosa which produces the

RBS Engineering

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1541017 short</partinfo>
 [[File:ETH_Zurich_2014_C4-HSL.png|thumb| ''N''-butyryl-L-Homoserine lactone or short C4-HSL ]]
-This part contains the part [[BBa_C0170]] (RhlI), which is an autoinducer synthesis protein that produces [[AHL|''N''-butyryl-L-HSL]] (C4-HSL) which binds to RhlR, ([[Part:BBa_C0171]]), obtained from ''Pseudomonas aeruginosa''. Since this part was used in a low-copy vector, RBS engineering was performed to increase expression levels of this enzyme and thus also improve the production of [[AHL|C4-HSL]].
+This part contains the part [[Part:BBa_C0170 C0170]] (RhlI), which is an autoinducer synthesis protein that produces [[AHL|''N''-butyryl-L-HSL]] (C4-HSL) which binds to RhlR, ([[Part:BBa_C0171 C0171]]), obtained from ''Pseudomonas aeruginosa''. Since this part was used in a low-copy vector, RBS engineering was performed to increase expression levels of this enzyme and thus also improve the production of [[AHL|C4-HSL]].
 ===Usage and Biology===