Difference between revisions of "Part:BBa K1317001"
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Linqing Li et Kristi L. Kiick. ''Resilin-Based Materials for Biomedical Applications'' (2013) ACS Macro Lett., 2(8), 635–640. doi: 10.1021/mz4002194.
 
Linqing Li et Kristi L. Kiick. ''Resilin-Based Materials for Biomedical Applications'' (2013) ACS Macro Lett., 2(8), 635–640. doi: 10.1021/mz4002194.
 +
 +
David H. Ardell and Svend Olav Andersen ''Tentative identification of a resilin gene in Drosophila melanogaster'' Insect Biochemistry and Molecular Biology 31 (2001) 965–970
 +
 +
Julie N. Renner et al. ''Characterization of Resilin-Based Materials for Tissue Engineering Applications'' Biomacromolecules 2012, 13, 3678−3685
 +
 +
Russell E. Lyons et al. ''Comparisons of Recombinant Resilin-like Proteins: Repetitive Domains Are Sufficient to Confer Resilin-like Properties'' Biomacromolecules 2009, 10, 3009–3014
 +
 +
M. Kim et al.  ''High yield expression of recombinant pro-resilin: Lactose-induced fermentation in E. coli and facile purification'' Protein Expression and Purification 52 (2007) 230–236
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Christopher M. Elvin et al. ''Synthesis and properties of crosslinked recombinant pro-resilin'' Vol 437|13 October 2005|doi:10.1038/nature04085

Revision as of 20:47, 16 October 2014

CDS for resilin-like polypeptide (RLP)

This part is the coding sequence for the resilin like polypeptide. This sequence was assembled from a consensus of the proresilin exon 1 from Drosophila melanogaster. This protein, in insects, allows resistance and elasticity used for jumping, flapping... The synthetic gene encodes a synthetic protein "resilin-like". The repeated aminoacids allow to retrieve these properties of resistance, resilience and elasticity, but with a minimal pattern of the original protein, which is more suited for downstream applications. It can be used to produce wire presenting these properties after wet-spinning.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 300
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Tamburro A.M. et al. Molecular and supramolecular structural studies on significant repetitive sequences of resilin (2010) Chembiochem., 11(1), 83-93. doi: 10.1002/cbic.200900460.

Linqing Li et Kristi L. Kiick. Resilin-Based Materials for Biomedical Applications (2013) ACS Macro Lett., 2(8), 635–640. doi: 10.1021/mz4002194.

David H. Ardell and Svend Olav Andersen Tentative identification of a resilin gene in Drosophila melanogaster Insect Biochemistry and Molecular Biology 31 (2001) 965–970

Julie N. Renner et al. Characterization of Resilin-Based Materials for Tissue Engineering Applications Biomacromolecules 2012, 13, 3678−3685

Russell E. Lyons et al. Comparisons of Recombinant Resilin-like Proteins: Repetitive Domains Are Sufficient to Confer Resilin-like Properties Biomacromolecules 2009, 10, 3009–3014

M. Kim et al. High yield expression of recombinant pro-resilin: Lactose-induced fermentation in E. coli and facile purification Protein Expression and Purification 52 (2007) 230–236

Christopher M. Elvin et al. Synthesis and properties of crosslinked recombinant pro-resilin Vol 437|13 October 2005|doi:10.1038/nature04085