Difference between revisions of "Part:BBa K1317001:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1317001 short</partinfo>
 
<partinfo>BBa_K1317001 short</partinfo>
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===Design Notes===
 
===Design Notes===
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The gene was synthesized with oligos specially designed for PCR fusion and Gibson Assembly.
 
  
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'''The gene used to assemble the biobrick has been provided by GenScript even if the assembling strategy succeeded'''
  
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It allows us to have the proper sequence without mutation and with sequencing results.
  
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The strategy of our assembly is described below anyway. The gene was synthesized by ordering specific oligo with overlapping regions, and by adding a NheI site for further fusing proteins.
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[[File:Bdx2014 RLPsynthesis1.png|900px]]
 
===Source===
 
===Source===
  
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Genomic sequence from Drosophila melanogaster
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Genomic sequence from ''Drosophila melanogaster''
  
 
===References===
 
===References===
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Julie N. Renner et al. ''Characterization of Resilin-Based Materials for Tissue Engineering Applications'' Biomacromolecules 2012, 13, 3678−3685
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Russell E. Lyons et al. ''Comparisons of Recombinant Resilin-like Proteins: Repetitive Domains Are Sufficient to Confer Resilin-like Properties'' Biomacromolecules 2009, 10, 3009–3014

Revision as of 20:39, 16 October 2014

CDS for resilin-like polypeptide (RLP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 300
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The gene used to assemble the biobrick has been provided by GenScript even if the assembling strategy succeeded

It allows us to have the proper sequence without mutation and with sequencing results.

The strategy of our assembly is described below anyway. The gene was synthesized by ordering specific oligo with overlapping regions, and by adding a NheI site for further fusing proteins.

Bdx2014 RLPsynthesis1.png

Source

Genomic sequence from Drosophila melanogaster

References

Julie N. Renner et al. Characterization of Resilin-Based Materials for Tissue Engineering Applications Biomacromolecules 2012, 13, 3678−3685

Russell E. Lyons et al. Comparisons of Recombinant Resilin-like Proteins: Repetitive Domains Are Sufficient to Confer Resilin-like Properties Biomacromolecules 2009, 10, 3009–3014