Difference between revisions of "Part:BBa K1351019"

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<partinfo>BBa_K1351019 short</partinfo>
 
<partinfo>BBa_K1351019 short</partinfo>
  
Artificial constructed complementary RNA which should bind the mRNA of the SdpI immunity protein in ''Bacillus subtilis'' in order to disturb the protein biosynthesis. Expected was a loss of immunity in b. subtilis against its own cannibalism toxin SdpABC over time. <br><br>
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Artificial constructed complementary RNA which should bind the mRNA of the SdpI immunity protein [https://parts.igem.org/Part:BBa_K1351017 (BBa_K1351017)] in ''Bacillus subtilis'' in order to disturb the protein biosynthesis. Expected was a loss of immunity in b. subtilis against its own cannibalism toxin SdpABC over time. <br><br>
 
Prefix: TGCAGAATTCGCGGCCGCTTCTAGAG<br>
 
Prefix: TGCAGAATTCGCGGCCGCTTCTAGAG<br>
 
Suffix: TACTAGTAGCGGCCGCTGCAGGCT
 
Suffix: TACTAGTAGCGGCCGCTGCAGGCT

Revision as of 20:13, 16 October 2014

Reverse complementary RNA sequence which binds the mRNA of the SdpI immunity

Artificial constructed complementary RNA which should bind the mRNA of the SdpI immunity protein (BBa_K1351017) in Bacillus subtilis in order to disturb the protein biosynthesis. Expected was a loss of immunity in b. subtilis against its own cannibalism toxin SdpABC over time.

Prefix: TGCAGAATTCGCGGCCGCTTCTAGAG
Suffix: TACTAGTAGCGGCCGCTGCAGGCT
Results: [http://2014.igem.org/Team:LMU-Munich/Results LMU-2014-Results]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]