Difference between revisions of "Part:BBa K1351019"
Line 2: | Line 2: | ||
<partinfo>BBa_K1351019 short</partinfo> | <partinfo>BBa_K1351019 short</partinfo> | ||
− | Artificial constructed complementary RNA which should bind the mRNA of the SdpI immunity protein in ''Bacillus subtilis'' in order to disturb the protein biosynthesis. Expected was a loss of immunity in b. subtilis against its own cannibalism toxin SdpABC over time. <br><br> | + | Artificial constructed complementary RNA which should bind the mRNA of the SdpI immunity protein [https://parts.igem.org/Part:BBa_K1351017 (BBa_K1351017)] in ''Bacillus subtilis'' in order to disturb the protein biosynthesis. Expected was a loss of immunity in b. subtilis against its own cannibalism toxin SdpABC over time. <br><br> |
Prefix: TGCAGAATTCGCGGCCGCTTCTAGAG<br> | Prefix: TGCAGAATTCGCGGCCGCTTCTAGAG<br> | ||
Suffix: TACTAGTAGCGGCCGCTGCAGGCT | Suffix: TACTAGTAGCGGCCGCTGCAGGCT |
Revision as of 20:13, 16 October 2014
Reverse complementary RNA sequence which binds the mRNA of the SdpI immunity
Artificial constructed complementary RNA which should bind the mRNA of the SdpI immunity protein (BBa_K1351017) in Bacillus subtilis in order to disturb the protein biosynthesis. Expected was a loss of immunity in b. subtilis against its own cannibalism toxin SdpABC over time.
Prefix: TGCAGAATTCGCGGCCGCTTCTAGAG
Suffix: TACTAGTAGCGGCCGCTGCAGGCT
Results: [http://2014.igem.org/Team:LMU-Munich/Results LMU-2014-Results]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]