Difference between revisions of "Part:BBa K1470006"

 
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Template pMIG
 
Template pMIG
  
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===Usage and Biology===
 
===Usage and Biology===
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<p> pMIG is a novel and easy to handle mammalian expression vector and to create fast and efficiently stable cell lines. It contains Long Terminal Repeats (LTR) for production of viral vectors. There is no packaging signal or gag, pol, env in pMIG. Thus it can be used in any S1 laboratory.
  
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Because of pMIGs retroviral origins, it can be used only in packaging cell lines such as Phoenix cells, to produce viral vectors. Otherwise there won’t be any functional vectors due to the lack of gag, pol, env and the psi-packaging signal.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<p>There is a multiple cloning site between the LTRs including EcoRI,PstI,BamHI and many more so you can insert any gene of interest. We suggest easy and reasonable digestion and ligation of an EcoRI- and PstI-site free plasmid backbone.
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<partinfo>BBa_K1470006 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1470006 SequenceAndFeatures</partinfo>
  

Revision as of 20:03, 16 October 2014

pMIG

Template pMIG


Usage and Biology

pMIG is a novel and easy to handle mammalian expression vector and to create fast and efficiently stable cell lines. It contains Long Terminal Repeats (LTR) for production of viral vectors. There is no packaging signal or gag, pol, env in pMIG. Thus it can be used in any S1 laboratory.

Because of pMIGs retroviral origins, it can be used only in packaging cell lines such as Phoenix cells, to produce viral vectors. Otherwise there won’t be any functional vectors due to the lack of gag, pol, env and the psi-packaging signal.

Sequence and Features

There is a multiple cloning site between the LTRs including EcoRI,PstI,BamHI and many more so you can insert any gene of interest. We suggest easy and reasonable digestion and ligation of an EcoRI- and PstI-site free plasmid backbone.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4917
    Illegal XbaI site found at 289
    Illegal XbaI site found at 3711
    Illegal PstI site found at 10
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4917
    Illegal NheI site found at 99
    Illegal NheI site found at 3521
    Illegal PstI site found at 10
    Illegal NotI site found at 4925
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4917
    Illegal BglII site found at 4899
    Illegal XhoI site found at 4905
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4917
    Illegal XbaI site found at 289
    Illegal XbaI site found at 3711
    Illegal PstI site found at 10
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4917
    Illegal XbaI site found at 289
    Illegal XbaI site found at 3711
    Illegal PstI site found at 10
    Illegal NgoMIV site found at 3466
    Illegal NgoMIV site found at 4123
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 520
    Illegal BsaI site found at 541
    Illegal BsaI site found at 3964
    Illegal BsaI.rc site found at 1901
    Illegal BsaI.rc site found at 4030
    Illegal BsaI.rc site found at 4645
    Illegal SapI site found at 818