Difference between revisions of "Part:BBa K1462630"

Revision as of 17:13, 16 October 2014

GAL1+GBD+SH3+SH3+SH3+PDZ+PDZ+PDZ+Tom22+ADH1

Scaffold protein, with a leading peptide Tom22, can locate on the outer mitochondria membrane with the scale of GBD: SH3: PDZ==1:3:3.We built up the scale to affirm the best scale. In this way , we tried to fix the PRK, RuBisCO, CA to a limited space to make full use of ATP and CO2 so that we coule improve the pyruvate offering to the butanol pathway.

<img src="">
Figure 1.This is the pathway and the structure of the carbon capture. As we see, with the help of ATP,the RuBiSco-5P change into RuBiSco-1,5P by PRK, and then change into 3P-glyrerate by RuBiSco, and CA can help us to adjust the concentration of carbon dioxide. The x,y,z are the number of scaffold unit.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 615
Illegal BamHI site found at 648
Illegal BamHI site found at 820
Illegal BamHI site found at 1029
Illegal BamHI site found at 1238
Illegal BamHI site found at 1447
Illegal BamHI site found at 1770
Illegal XhoI site found at 801
Illegal XhoI site found at 1010
Illegal XhoI site found at 1219
Illegal XhoI site found at 1428
Illegal XhoI site found at 1751
Illegal XhoI site found at 2074
Illegal XhoI site found at 2397
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 150
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1462630 short</partinfo>
-df
+Scaffold protein, with a leading peptide Tom22, can locate on the outer mitochondria membrane with the scale of GBD: SH3: PDZ==1:3:3.We  built up the scale to affirm the best scale. In this way , we tried to fix the PRK, RuBisCO, CA  to a limited space to make full use of ATP and CO2 so that we coule improve the pyruvate offering to the butanol pathway.
+<img src="https://static.igem.org/mediawiki/parts/f/fb/Rubisco.jpg"><br/>
+Figure 1.This is the pathway and the structure of the carbon capture. As we see, with the help of ATP,the RuBiSco-5P change into RuBiSco-1,5P by PRK, and then change into 3P-glyrerate by RuBiSco, and CA can help us to adjust the concentration of carbon dioxide. The x,y,z are the number of scaffold unit.
 <!-- Add more about the biology of this part here