Difference between revisions of "Part:BBa K1462580"

Revision as of 16:53, 16 October 2014

GAL1+GBD+SH3+SH3+PDZ+PDZ+Tom22+ADH1

Scaffold protein, with a leading peptide Tom22, can locate on the outer mitochondria membrane with the scale of GBD: SH3: PDZ==1:2:2.We built up the scale to affirm the best scale. In this way , we tried to fix the PRK, RuBisCO, CA to a limited space to make full use of ATP and CO2 so that we coule improve the pyruvate offering to the butanol pathway.

<img src="">
Figure 1.This is the pathway and the structure of the carbon capture. As we see, with the help of ATP,the RuBiSco-5P change into RuBiSco-1,5P by PRK, and then change into 3P-glyrerate by RuBiSco, and CA can help us to adjust the concentration of carbon dioxide. The x,y,z are the number of scaffold unit.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 2082
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 2082
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 615
Illegal BamHI site found at 648
Illegal BamHI site found at 820
Illegal BamHI site found at 1029
Illegal BamHI site found at 1238
Illegal BamHI site found at 1561
Illegal BamHI site found at 1884
Illegal XhoI site found at 801
Illegal XhoI site found at 1010
Illegal XhoI site found at 1219
Illegal XhoI site found at 1542
Illegal XhoI site found at 1865
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 2082
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 2082
Illegal AgeI site found at 150
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K1462580 short</partinfo>
-ccc
+Scaffold protein, with a leading peptide Tom22, can locate on the outer mitochondria membrane with the scale of GBD: SH3: PDZ==1:2:2.We  built up the scale to affirm the best scale. In this way , we tried to fix the PRK, RuBisCO, CA  to a limited space to make full use of ATP and CO2 so that we coule improve the pyruvate offering to the butanol pathway.
+<img src="https://static.igem.org/mediawiki/parts/f/fb/Rubisco.jpg"><br/>
+Figure 1.This is the pathway and the structure of the carbon capture. As we see, with the help of ATP,the RuBiSco-5P change into RuBiSco-1,5P by PRK, and then change into 3P-glyrerate by RuBiSco, and CA can help us to adjust the concentration of carbon dioxide. The x,y,z are the number of scaffold unit.
 <!-- Add more about the biology of this part here