Difference between revisions of "Part:BBa K1378003"
(→Usage and Biology) |
|||
Line 27: | Line 27: | ||
</html> | </html> | ||
+ | =='''Reference'''== | ||
+ | <p>1.Kehr, J. C., Zilliges, Y., Springer, A., Disney, M. D., Ratner, D. D., Bouchier, C., ... & Dittmann, E. (2006). A mannan binding lectin is involved in cell–cell attachment in a toxic strain of Microcystis aeruginosa. Molecular microbiology, 59(3), 893-906.</p> | ||
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K1378003 parameters</partinfo> | <partinfo>BBa_K1378003 parameters</partinfo> | ||
<!-- --> | <!-- --> |
Revision as of 16:52, 16 October 2014
Microvirin (MVN)---a lectin from Microcystis aeruginosa
Introduction
This plasmid is a basic part of the mannan binding lectin Microvirin (MVN). Microvirin can be expressed in E. coli and it can bind to Microcystis aeruginosa PCC7806 specifically.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 23
Usage and Biology
MVN is a lectin isolated from the cyanobacteria Microcystis aeruginosa PCC7806. It is an 108 aa protein that consists of 2 domains. According to a carbohydrate microarray carried out previously, MVN binds to carbohydrate and the highest signals are observed with structures that contain α(1→2) linked mannose residues [1]. A binding partner of MVN was identified in the lipopolysaccharide (LPS) fraction of M. aeruginosa PCC7806 and it possibly represents the O-antigen of a LPS. MVN has been previously expressed in E. coli and its binding effect to cyanobacteria cells is species-specific [1].
Reference
1.Kehr, J. C., Zilliges, Y., Springer, A., Disney, M. D., Ratner, D. D., Bouchier, C., ... & Dittmann, E. (2006). A mannan binding lectin is involved in cell–cell attachment in a toxic strain of Microcystis aeruginosa. Molecular microbiology, 59(3), 893-906.